Fig. 6: Comparison of visualisation methods including deconvolution. | Communications Biology

Fig. 6: Comparison of visualisation methods including deconvolution.

From: A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci

Fig. 6

This compares the number of foci detected for the four radiation types delivering 1 Gy at two-time points. Where a and b is 30 min and 24 h post-irradiation respectively. The actual value is given by the blue bar which is the known simulated number of DSBs within the evaluated z-slice. Whereas, the other bars represent the foci detected from the corresponding visualisation method. Marker visualisation which has been deconvolved represent perfect deconvolution as the image is created before the microscope PSF is applied. Each bar is the averaged result of 200 independent samples and the error bars are the 95% confidence intervals. All data are for an emulated Airyscan ×63 microscope.

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