Fig. 7: Percentage foci miscount when using a single slice and 3D stack microscopy. | Communications Biology

Fig. 7: Percentage foci miscount when using a single slice and 3D stack microscopy.

From: A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci

Fig. 7

This compares the number of foci detected for the four radiation types delivering 1 Gy at two-time points. Where a and b is 30 min and 24 h post-irradiation respectively. The percentage difference is calculated as the \(\left({{{{{{\rm{counted}}}}}}}-{{{{{{\rm{simulated}}}}}}}\right./(({{{{{{\rm{counted}}}}}}}+{{{{{{\rm{simulated}}}}}}})\times 0.5)\times 100\). 3D foci counting consistently under-counts foci at the 30 min time point with its magnitude increasing as a function of LET. Whereas, 3D foci counting appears to correctly count the small number of foci left at 24 h with an exception of consistent under-counting at high LET. Each bar is the averaged result of 50 independent samples and the error bars are the 95% confidence intervals. All data are for an emulated Airyscan ×63 microscope.

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