Table 1 Microscope parameters—the pixel spacing for XY and Z (μm) that the resultant PyFoci microscope image uses.

From: A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci

Microscope parameters (XY pixel spacing, Z pixel spacing, numerical aperture)

 

×10

×20

×40

×63

×100

Airyscan

0.13, 1.14, 0.45

0.059, 0.3, 0.8

0.035, 0.15, 1.3

0.033, 0.12, 1.4

0.028, 0.1, 1.46

gSTED

–

0.065, 0.3, 0.95a

0.055, 0.20, 1.1

0.042, 0.13, 1.4

0.44, 0.13, 1.4

Lowlight

–

0.21, 0.5, 0.5

0.11, 0.3, 0.75

–

0.043, 0.1, 1.45

MultiPhoton

0.20, 1.0, 0.3

0.54, 0.25, 0.95b

0.058, 0.2, 0.85

0.044, 0.15, 1.2

–

Phenix

–

0.3, 0.8, 1.0

0.15, 0.5, 1.1

0.095, 0.4, 1.15

–

STED

–

0.075, 0.4, 0.75

0.045, 0.15, 1.1

0.034, 0.1, 1.4

0.035, 0.1, 1.4

  1. Along with the numerical aperture used when measuring the microscope PSF. Values given for all size microscopes at their respective magnifications. The STED microscope also has a PSF at ×25 which has the following values (0.06, 0.25, 0.95) for XY pixel spacing, Z pixel spacing and numerical aperture respectively.
  2. a×23 magnification not ×20.
  3. b×25 magnification not ×20.
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