Fig. 2: Identification of LT-HSC and MPP2 as niche for viable parasites during infection and following treatment failure. | Communications Biology

Fig. 2: Identification of LT-HSC and MPP2 as niche for viable parasites during infection and following treatment failure.

From: Long-term hematopoietic stem cells as a parasite niche during treatment failure in visceral leishmaniasis

Fig. 2

a Specific markers for BM cell subsets: long-term hematopoietic stem cell (LT-HSC), short-term HSC (ST-HSC), multipotent progenitor (MPP), common myeloid progenitor (CMP), granulocyte-monocyte progenitor (GMP), mesenchymal stem cells (MSC). b Cell sorting of DsRed+ BM cells of LEM3323 WTPpyRE9/DsRed infected BALB/c mice to enrich the infected sample, followed by re-measuring with markers as described in (a) and gated on LSK cells, i.e., Lin−, Sca-1+ and cKit+ cells. Fluorescence minus one (FMO) controls are displayed to confirm the gates. Representative plots and frequencies of DsRed+ cells (mean ± SD) are shown of two independent experiments per condition (n = 4), performed at 4 wpi (infected) and 5 wpt (relapsed). c Giemsa-stained LT-HSC and MPP2 cells sorted from LEM3323 WTPpyRE9/DsRed infected BALB/c mice (6wpi). Scale bar = 10 µm. d Results of promastigote back-transformation assays after FACS of an indicated number of cells (LT-HSC, ST-HSC, MPP2, MPP3, CMP/GMP, and Lin+ cells) from infected untreated mice (top panel, pooled results of 4, 5 and 6 wpi) and relapsed mice after PMM treatment (bottom panel, pooled results of 3, 4 and 5 wpt). Colored bars represent independent experiments (3 ≤ n ≤ 5) and positive back-transformation. Statistical significance levels using titration endpoints are indicated (2-way ANOVA, multiple comparisons). *p < 0.05, ***p < 0.001, ****p < 0.0001. The diagram in (a) was generated using Biorender.com.

Back to article page