Fig. 4: Altered neuronal growth is through neuron-intrinsic functions of KHSRP.
a Representative epifluorescent images of distal dendrites for DIV23 cultures of LV-GFP transduced neocortical neurons from E18 Khsrp+/+ and Khsrp−/− mice shown as indicated. b Quantification of dendritic spine density and morphology for cortical neurons as in a is shown (N > 30 neurons in at least 3 separate cultures; *p ≤ 0.05 and **p ≤ 0.01 by Student’s t test). c Representative epifluorescent images of distal dendrites for DIV23 cortical neuron cultures immunostained for GFP (magenta) and synaptophysin (gray). d Quantification of synapse density and % colocalization of pre- and post-synaptic markers for cortical neurons as in c is shown (N ≥ 30 neurons in at least 3 separate culture preparations; ***p ≤ 0.005 and ****p ≤ 0.001 by Student’s t test). e, f Quantification of Khsrp mRNA by RTddPCR and KHSRP and GAP43 proteins by immunofluorescence in E18 neocortical neurons cultured from Khsrpfl/fl and Khsrpfl/fl × Syn1:Cre mice is shown (N ≥ 30 neurons in at least 3 separate culture preparations; ****p ≤ 0.001 by Student’s t test). g, h Quantitation of axon and dendrite length (g) and branching (h) for neocortical neurons from E18 Khsrpfl/fl and Khsrpfl/fl × Syn1:Cre mice shown as indicated. Axons were identified by immunostaining for SMI312 and dendrites by Tau as in Fig. 3 (N ≥ 60 neurons over 3 separate culture preparations; **p ≤ 0.01 by Student’s t test).
