Fig. 7: RNASE4 mAb inhibits the establishment of xenograft human prostate cell tumors in athymic mice.
From: Ribonuclease 4 is associated with aggressiveness and progression of prostate cancer
PC-3 cells (1 × 106 per mouse) were inoculated s.c. on the back of male athymic mice. One day post-inoculation, mice were treated by i.p. injection with PBS (n = 6) or RNASE4 mAb (10 mg/kg, n = 6) once every 3 days. a Tumor volume measured once every 3 days. b Animals were sacrificed on day 64, tumors were dissected and weighed. Left panel, tumor weight; right panel, representative images of tumors from PBS- and RANSE4 mAb-treated groups. c Body weight of PBS- and RNASE4 mAb-treated animals. d IHC analyses of Ki-67, CD31, total AXL, and phophoylated AXL (p-AXL) in tumor sections from PBS- and RNASE4 mAb-treated animals. Left panels, representative images; right panels, quantification of Ki-67 positive cells and CD31 positive neovessels from 5 randomly selected microscopic fields. e TUNEL staining of apoptotic cells. Nuclei were stained by DAPI. Right panels, representative images; right panel, percentage of TUNEL positive cells counted in five randomly selected areas. Data shown are means ± SEM. Scale bars = 100 µm. **p ≤ 0.01, ***p ≤ 0.001, and n.s. = not significant, by two-tailed Student’s t test.
