Fig. 8: RNASE4 mAb inhibits the growth of established xenograft human prostate cell tumors in athymic mice.
From: Ribonuclease 4 is associated with aggressiveness and progression of prostate cancer
PC-3 cells (1 × 106) were inoculated s.c. on the back of male athymic mice. After tumors reached ~100 mm3 in volume (day 21), mice were grouped and treated by i.p. injection of PBS (n = 12) or RNASE4 mAb (10 mg/kg or 30 mg/kg, n = 6 per group) three times per week. a Tumor volume measured every 3 days. b Tumor weight. Mice were sacrificed on day 39, tumors were dissected and weighed. Left panel, average tumor weight; right panel, representative tumor images from the three groups. c IHC analyses for ki-67, CD31, total AXL, and phosphorylated AXL (p-AXL). Left panels, representative images; right panels, quantification of Ki-67 positive cells and CD31 positive neovessels from 5 randomly selected microscopic fields. d TUNEL staining of apoptotic cells. Nuclei were stained by DAPI. Right panels, representative images; right panel, percentage of TUNEL positive cells in five randomly selected areas. Data shown are means ± SEM. Scale bars = 100 µm. *p ≤ 0.05 and **p ≤ 0.01, by two-tailed Student’s t test.
