Fig. 1: ACE2 expression is upregulated in intestinal cells response to amino acid deficiency. | Communications Biology

Fig. 1: ACE2 expression is upregulated in intestinal cells response to amino acid deficiency.

From: Amino acid sensor GCN2 promotes SARS-CoV-2 receptor ACE2 expression in response to amino acid deprivation

Fig. 1

a CCD841 cells were incubated with control culture medium (Con) or essential amino acid (leucine, LEU; Valine, VAL; Isoleucine, ILE; Threonine, THR; Lysine, LYS; Tryptophan, TRP; Phenylalanine, PHE; Methionine, MET; Histidine, HIS) deprived culture medium for 48 h. ACE2 and p-EIF2A expression were analyzed by western blotting (left) and quantified by densitometric analysis (right). b, c 10-week-old male wild type mice were fed with control diet (NCD) or leucine deprivation diet (LDD) for 7 days. ACE2 mRNA and protein levels in colonic epithelial cells were analyzed by qRT-PCR and western blotting, respectively. Protein levels were quantified by densitometric analysis (right); A.U.: arbitrary unit. Data are expressed as the mean ± SEM (n = 5–6 per group, as indicated by scatter circles). *P < 0.05, **P < 0.01.

Back to article page