Fig. 2: GCN2 regulates ACE2 expression in vitro and in vivo. | Communications Biology

Fig. 2: GCN2 regulates ACE2 expression in vitro and in vivo.

From: Amino acid sensor GCN2 promotes SARS-CoV-2 receptor ACE2 expression in response to amino acid deprivation

Fig. 2

a, b CCD841 cells were transfected with control small interfering RNAs (siNC) or small interfering RNAs targeting at human GCN2 (siGCN2) for 48 h. mRNA expression of GCN2 and ACE2 were analyzed by qRT-PCR (a); GCN2, p-EIF2A, and ACE2 protein levels were analyzed by western blotting (left) and quantified by densitometric analysis (right); A.U.: arbitrary unit (b); c, d small intestinal and colonic epithelial cells isolated from 10-week-old male Gcn2-floxed mice (Con) or intestinal epithelial cell (IEC)-specific Gcn2 deletion mice (Gcn2 IKO). mRNA expression of Gcn2 and Ace2 were analyzed by qRT-PCR (c); GCN2 and ACE2 protein levels were analyzed by western blotting (top) and quantified by densitometric analysis (bottom) (d). e CCD841 cells were incubated with indicated concentration GCN2iB (HY-112654) for 48 h, then ACE2 protein levels were analyzed by western blotting (left) and quantified by densitometric analysis (right). Data are expressed as the mean ± SEM (n = 3–6 per group, as indicated by scatter circles). *P < 0.05, **P < 0.01.

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