Fig. 2: Mitochondria show significant fragmentation in MEFs from RAB7^V162M mutant mice.

Mouse embryonic skin fibroblasts from + /+, fl/+, fl/fl E18 mice were dissected, cultured and maintained as described in Materials and Methods. MEFs were incubated with Mito Tracker and images were captured by live-cell imaging. a–c: representative images of mitochondria in + /+, fl/+, fl/fl. To better illustrate the mitochondria morphology, a small inset from each image (white box) was magnified and presented. The images were analyzed and quantitated using the Mitochondria Analyzer Plugin in Fiji (ImageJ). The measurements for mean area (d), mean perimeter (e), aspect ratio (f), form factor (g), the number of branch junctions/mitochondrion (h), the number of branches/mitochondrion (i), total branch length (j) and mean branch length (k) are presented. In addition, histogram distributions of mitochondria with respect to these measurements are also included in each perspective parameter. Results are shown as mean ± SEM. The numbers of mitochondria were analyzed are: n = 20350 from 46 images (~100 cells) for + /+, n = 22947 from 40 images (~85 cells) for fl/+, n = 28936 from 47 images (~100 cells) for fl/fl. Significance analysis was carried out using Prism. Statistical significances were calculated by One-Way ANOVA. All p values are shown in the graphs.