Fig. 3: Mitochondrial fragmentation in fl/fl MEFs is rescued by both Mdivi-1 and CID1067700 treatment.

MEFs from fl/fl E18 embryos were cultured and were treated DMSO, 50 µM Mdivi-1, 1 µM CID1067700, 10 µM CID1067700. Mitochondria labeling, live cell imaging and mitochondrial quantitation were as in Fig. 2. a–d: Representative images for cells treated with DMSO, 50 µM Mdivi-1, 1 µM CID1067700, 10 µM CID1067700. To better illustrate the mitochondria morphology, a small inset from each image (white box) was magnified and presented. The measurements for mean Area (e), mean perimeter (f), aspect ratio (g), form factor (h), the number of branch junctions/mitochondrion (i), the number of branches/mitochondrion (j), total branch length (k) and mean branch length (l) are presented. In addition, histogram distributions of mitochondria with respect to these measurements are also included in each perspective parameter. Results are shown as mean ± SEM. The numbers of mitochondria were analyzed are: n = 28050 from 34 images (~65 cells) for DMSO, n = 23251 from 39 images (~75 cells) for Mdivi-1, n = 22628 from 36 images for 1 µM CID1067700, n = 28247 from 40 images (~90 cells) for 10 µM CID1067700. Significance analysis was carried out using Prism. Statistical significances were calculated by One-Way ANOVA. All p values are shown in the graphs.