Fig. 7: The inhibition of PI3Kδ activity increases albumin protein level in different hepatic cell lines. | Communications Biology

Fig. 7: The inhibition of PI3Kδ activity increases albumin protein level in different hepatic cell lines.

From: PI3Kδ activity controls plasticity and discriminates between EMT and stemness based on distinct TGFβ signaling

Fig. 7

a Immunofluorescence staining for Albumin (green), actin microfilaments using phalloidin (red) and nuclei (blue) in HepG2, Hep3B and HepaRG cells plated in 3D cultures for 6 days and treated or not with the PI3Kδ specific inhibitor (CAL-101) at different doses. Scale bar: 10 µm. b Quantification of albumin relative intensity in the different cell lines treated or not with CAL-101 at different doses. c Experiment plan for HepaRG differentiation using DMSO or CAL-101. d Immunofluorescence staining for Albumin (green), actin microfilaments using phalloidin (red) and nuclei (blue) in HepaRG cells treated with DMSO or CAL-101 at different doses after 7 days of treatment. Scale bar: 100 µm. Quantification of albumin relative intensity in HepaRG within the different conditions above. e Proposed PI3Kδ dependant pathway discriminating EMT from stemness. All values are expressed as mean ± S.E.M.

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