Fig. 5: Generation of SANPs-siRNA targeting praja2. | Communications Biology

Fig. 5: Generation of SANPs-siRNA targeting praja2.

From: Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth

Fig. 5

a Schematic representation of self-assembling nanoparticles (SANPs) conjugated with transferrin and encapsulating control siRNA or siRNA targeting praja2. b Cells were perfused for 72 h with different preparations of SANPs-siRNAs targeting praja2 (F1: SANP1-siPraja2; F2: SANP2-siPraja2; F3: SANP3-siPraja2; F4: SANP4-siPraja2; F5: SANP5-siPraja2; F6: SANP6-siPraja2) or control SANPs (SANPs-siRNAc). Lysates were immunoblotted with anti-praja2 and anti-HSP90 antibodies. c Quantitative analysis of the experiments shown in panel b. Reported data were the mean values ± SEM of three independent experiments. P value: * = 0.0167; * = 0.0169. d Growth curves of U87MG cells treated with vehicle, SANPs-siRNAc, SANPs-siPraja2. At indicated time points, cells were harvested and counted. Three independent experiments were performed and the corresponding mean values ± SEM are shown. P value: ** = 0.0054. e, f FACS analysis of U87MG cells treated with siRNAc or siPraja2 (e), and SANPs-siRNAc or SANPs-siPraja2 (f) for 72 h. Cell cycle distribution (G0/G1, S, and G2/M) of treated cells is indicated as a percentage of total cells scored.

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