Fig. 1: Growth inhibition of Mtb upon treatment with sAEL057 in various conditions.

a, b Mtb were grown in broth (7H9 + OADC, 7H9 + Oleate, 7H9 + Acetate, 7H9 + Glucose, and 7H9 + Cholesterol) and treated with sAEL057 at 50 µM down to 0.097 µM in a dose curve-dependent manner. Alamar blue was added on day 9 and results were measured via fluorescence on day 10. HMDMs were infected at MOI 2:1 with mCherry-expressing Mtb and fluorescence values were measured at 7dpi. a Representative graph of percent inhibition curves. Percent inhibition was determined relative to DMSO (0% inhibition) and 20 µM RIF (100% inhibition) controls. n = 2 technical replicates from a representative experiment. b EC₅₀ calculated using nonlinear regression analyses (log inhibitor vs response) of percent inhibition curves. n = 3–5 replicate experiments c, d Mtb was grown in 7H9 + OADC (c) or 7H9 + cholesterol (d), and replicate plates were used for plating CFUs on day 5 and day 10 posttreatment. n = 4 from two replicate experiments. e Mtb-infected HMDMs were lysed via 0.01% SDS in water at 4 dpi and 8 dpi to plate for CFU. n = 4 from two replicate experiments. Statistical significance and P values were assessed using a student’s unpaired t-test. Error bars indicate standard deviation. Source data and P values for all main figures are available in Supplementary Data 1.