Fig. 3: Gallium addition synergizes with sAEL057 treatment to inhibit Mtb growth in cholesterol media but not in rich broth.
From: Iron limitation in M. tuberculosis has broad impact on central carbon metabolism
Mtb were grown in cholesterol-supplemented media (a–c) or rich broth (d–f) and treated with sAEL057 at 50 µM down to 0.097 µM in a dose curve-dependent manner. Alamar blue was added at day 9 and results were measured via fluorescence on day 10. a, d Representative graphs of percent inhibition curves. Percent inhibition was determined relative to DMSO (0% inhibition) and 20 µM RIF (100% inhibition) controls. n = 2 technical replicates from a representative experiment. b, e EC50 values calculated using nonlinear regression analyses (log inhibitor vs response) of percent inhibition curves. n = 4 from two replicate experiments. c, f % Fitness defect was calculated by dividing gallium only (without sAEL057) controls by DMSO controls and multiplying by 100 in the given media condition. n = 4–6 from two replicate experiments. Statistical significance was assessed using a student’s unpaired t-test. Error bars indicate standard deviation. Source data and P values for all main figures are available in Supplementary Data 1.
