Fig. 1: LncRNA-CIRPIL is downregulated in ischemia/reperfusion (I/R) hearts and anoxia/reoxygenation (A/R) NMCMs.

a The flowchart of study of I/R-induced expression profiles of murine lncRNA by MTA 1.0 transcriptome microarray assay. b The volcano plots of the I/R-induced expression profiles of murine lncRNAs in ischemia zone (IZ) compared with non-ischemia zone (NIZ) and border zone (BZ). n = 3. c Analysis of dysregulated lncRNAs in the hearts of I/R mice based on microarray profiling. d Validation of the heart-enriched and with more than 1.5 folds change intergenic lncRNAs in the hearts of I/R mice. n = 5. *P < 0.05 vs sham by two-tailed Student’s t test. e The expression changes of lncCIRPIL in NMCMs exposed to A/R. n = 18 samples from 6 independent experiments. *P < 0.05 vs control by two-tailed Student’s t test. f. Subcellular localization of lncCIRPIL in NMCMs and adult mouse cardiomyocytes detected by Fluorescent in situ hybridization (FISH) & Immunofluorescence (IF) after A/R or I/R injury. n = 3. Scale bar = 20 μm. DAPI indicates 4′,6-diamidino-2-phenylindole.