Fig. 2: Effects of lncCIRPIL on anoxia/reoxygenation(A/R) induced injury of NMCMs.
From: Cytoplasmic sequestration of p53 by lncRNA-CIRPILalleviates myocardial ischemia/reperfusion injury
a Validation of lncCIRPIL overexpression plasmid in NMCMs. n = 3. *P < 0.05 vs NC (negative control) by two-tailed Student’s t test. b Knockdown efficiency of shRNAs for lncCIRPIL in NMCMs. n = 3. *P < 0.05 vs NC by two-tailed Student’s t test. c Effects of lncCIRPIL on LDH release of NMCMs subjected to A/R insult. n = 4. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. d Effects of lncCIRPIL on cell apoptosis of NMCMs subjected to A/R insult by TUNEL staining. n = 18 slices from 3 independent experiments for each group. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis; Scale bar = 50 μm. e Effects of lncCIRPIL on Bcl2/Bax ratio of NMCMs subjected to A/R insult. n = 4. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. f Effects of lncCIRPIL on caspase-3, caspase-8, caspase-9 activities of NMCMs subjected to A/R insult. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis.
