Fig. 3: Cardiomyocyte-specific overexpression of lncCIRPIL protects the heart from ischemia/reperfusion (I/R) injury in mice.

a LncCIRPIL expression in major organs of WT and lncCIRPIL-TG mice. n = 3. *P < 0.05 vs WT by two-tailed Student’s t test. b Representative cardiac echocardiography and statistical analysis of cardiac function of WT and lncCIRPIL transgenic (TG) mice subjected to cardiac I/R injury. EF eject fraction, FS fractional shortening. n = 9 to 11. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. c, d Representative images of heart sections of WT and lncCIRPIL-TG mice after I/R injury by TTC and EVANS blue staining, and statistical analysis of at-risk area (AAR) and infarct area (IA) to AAR ratio. n = 9 to 10. *P < 0.05 vs WT + I/R by 2-tailed Student t test. scale bar = 2 mm. e Serum CK-MB in WT and lncCIRPIL-TG mice after cardiac I/R injury. n = 6 to 9. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. f Serum LDH in WT and lncCIRPIL-TG mice after cardiac I/R injury. n = 6 to 11 mice. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. g Caspase3 activity in cardiac tissue of WT and lncCIRPIL-TG mice subjected to I/R injury. n = 9. *P < 0.05 by one-way ANOVA, followed by Tukey post hoc analysis. h Effects of lncCIRPIL on apoptosis of heart tissue from mice subjected to I/R insult by TUNEL staining. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis; Scale bar = 50 μm. i Bcl2/Bax ratio in cardiac tissues of WT and lncCIRPIL-TG mice subjected to I/R injury. n = 5. *P < 0.05 by one-way ANOVA, followed by Tukey post hoc analysis.