Fig. 4: Knockout of lncCIRPIL exacerbates cardiac ischemia/reperfusion (I/R) injury in mice.

a The expression of LncCIRPIL in heart tissue of WT and lncCIRPIL-KO mice. n = 6. *P < 0.05 vs WT by two-tailed Student’s t test. b Representative cardiac echocardiography and statistical analysis of cardiac function of WT and lncCIRPIL-KO mice subjected to cardiac I/R injury. EF, eject fraction; FS, fractional shortening. n = 9 to 11. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. c, d Representative images of heart sections from WT and lncCIRPIL-KO mice after I/R injury by TTC and EVANS blue staining, and statistical analysis of at-risk area (AAR) and infarct area (IA) to AAR ratio. n = 10. *P < 0.05 vs WT + I/R by two-tailed Student’s t test. Scale bar = 2 mm. e Serum CK-MB in WT and lncCIRPIL-KO mice after cardiac I/R injury. n = 9 or 10. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. f Serum LDH in WT and lncCIRPIL-KO mice after cardiac I/R injury. n = 9 to 16. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. g The caspase3 activity in cardiac tissue of WT and lncCIRPIL-KO mice subjected to I/R injury. n = 9. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. h Bcl2/Bax ratio in cardiac tissue of WT and lncCIRPIL-KO mice subjected to I/R injury. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis.