Fig. 5: LncCIRPIL binds with p53.
From: Cytoplasmic sequestration of p53 by lncRNA-CIRPILalleviates myocardial ischemia/reperfusion injury
a Silver staining of electrophoretic separated proteins pulled down by sense and antisense sequences of lncCIRPIL. Mass spectrometry was performed on the band specifically pulled down by sense sequence and p53 was identified. b Western blot of p53 pulled down by sense sequence of lncCIRPIL. n = 3. c LncCIRPIL immunoprecipitated by anti-p53 antibody. n = 3. *P < 0.05 vs IgG by two-tailed Student’s t test. d Western blot of p53 pulled down by biotin-labeled fragments of lncCIRPIL. n = 3. e Effects of fragments of lncCIRPIL on LDH release of NMCMs subjected to anoxia/reoxygenation(A/R) insult. n = 6 samples from 3 independent experiments for each group. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. f Western blot of flag-labeled fragments of p53 pulled down by sense sequence of lncCIRPIL. n = 3. g LncCIRPIL immunoprecipitated by anti-flag antibody for flag-labeled fragments of p53. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis.
