Fig. 6: LncCIRPIL promotes the ubiquitin-mediated degradation of p53 during cardiac injury.
From: Cytoplasmic sequestration of p53 by lncRNA-CIRPILalleviates myocardial ischemia/reperfusion injury
a Influence of lncCIRPIL overexpression on cytoplasmic and nuclear distribution of p53 in NMCMs subjected to A/R insult by immunofluorescence staining. n = 3. Scale bar = 20 μm. b Effects of lncCIRPIL overexpression on total, cytoplasmic and nuclear levels of p53 in NMCMs subjected to anoxia/reoxygenation (A/R) insult. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. c Effect of lncCIRPIL on the mRNA level of p53 in NMCMs subjected to A/R insult. n = 9 samples from 3 independent experiments. *P < 0.05 by one-way ANOVA, followed by Tukey post hoc analysis. d Effects of lncCIRPIL overexpression on the ubiquitination of p53 in NMCMs subjected to A/R insult. n = 4. e. Blocking of protein degradation by MG132 leads to cytoplasmic accumulation of p53 in lncCIRPIL overexpressing NMCMs subjected to A/R insult. n = 4. Scale bar = 20 μm. f The shRNAs of CHIP, MDM2, COP1, HUWE1 and RCHY1 on the protein level of p53 in NMCMs subjected to A/R insult. n = 3. *P < 0.05 vs NC, #P < 0.05 vs A/R + NC, ΔP < 0.05 vs A/R + lncCIRPBL(OE) by one-way ANOVA followed by Tukey post hoc analysis. g Effects of lncCIRPIL overexpression on the transcription of p53-downstream genes Bax Puma and Noxa. n = 9 samples for Bax and Puma, n = 6 samples for Noxa from 3 independent experiments. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis.
