Fig. 8: The effects of human conserved fragment of lncCIRPIL (hcf-lncCIRPIL) on human AC16 cells exposed to A/R insult.
From: Cytoplasmic sequestration of p53 by lncRNA-CIRPILalleviates myocardial ischemia/reperfusion injury
a Western blot of p53 pulled down by sense sequence of hcf-lncCIRPIL in human AC16 cells. n = 3. b The level of hcf-lncCIRPIL in AC16 cells transfected with hcf-lncCIRPIL overexpression plasmid. n = 3. *P < 0.05 vs NC by two-tailed Student’s t test. c, d Effects of hcf-lncCIRPIL overexpression on Bcl2/Bax ratio and p53 level in AC16 cells exposed to A/R insult. n = 5. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis. e Effects of hcf-lncCIRPIL overexpression on p53 cellular distribution. n = 3. Scale bar = 20 μm. f. Knockdown efficiency of hcf-lncCIRPIL by the antisense oligonucleotide (ASO). n = 3. *P < 0.05 vs NC by two-tailed Student’s t test. g, h Effects of hcf-lncCIRPIL-ASO on Bcl2/Bax ratio and p53 level in AC16 cells exposed to A/R insult by. n = 3. *P < 0.05 by one-way ANOVA followed by Tukey post hoc analysis.
