Table 1 Detailed information for key resources and fly stocks.

From: A male-specific doublesex isoform reveals an evolutionary pathway of sexual development via distinct alternative splicing mechanisms

Reagent type (species) or resource

Designation

Source or reference

Identifiers

Additional information

Antibody

Mouse monoclonal anti-Flag

Sigma–Aldrich

Cat# F1804

IHC (1:500)

Antibody

Mouse monoclonal anti-Myc

MBL

M047-3

IHC (1:200)

Antibody

Donkey polyclonal anti-Mouse, Alexa Fluor 488

Thermo Fisher Scientific

Cat# A-21202, RRID: AB_141607

IHC (1:500)

Antibody

Donkey polyclonal anti-Mouse, Alexa Fluor 555

Thermo Fisher Scientific

Cat# A-31570, RRID: AB_2536180

IHC (1:500)

Plasmid

pVALIUM20

Tsinghua University

  

Plasmid

pJFRC2-10×UAS-IVS-mCD8::GFP

Addgene

#26214

 

Chemical compound drug

Normal Goat Serum (NGS)

Jackson ImmunoResearch Laboratories

Code# 005-000-121 RRID: AB_2336990

3% NGS in 1×PBS

Chemical compound drug

Paraformaldehyde (PFA)

Sigma–Aldrich

CAS# 30525-89-4

4% PFA in 1×PBS

Chemical compound drug

TRIzolâ„¢ reagent

Invitrogen

Cat# 15596026

 

Chemical compound drug

SuperScriptâ„¢ IV

Invitrogen

Cat# 18091050

 

Chemical compound drug

DNA Polymerase High Fidelity

Transgen

Cat# AS131-21

 

Chemical compound drug

EvaGreen Dye

Biotium

Cat# 31000

 

Genetic reagent (D. melanogaster)

UAS-dsxMRNAi

14

  

Genetic reagent (D. melanogaster)

dsxGAL4

12

  

Genetic reagent (D. melanogaster)

dsxGAL4(Δ2)

36

  

Genetic reagent (D. melanogaster)

R57C10-GAL4

Bloomington Drosophila Stock Center

BDSC_39171

 

Genetic reagent (D. melanogaster)

actin-GAL4

Bloomington Drosophila Stock Center

BDSC_25374

 

Genetic reagent (D. melanogaster)

UAS-traRNAi

Bloomington Drosophila Stock Center

BDSC_28512

 

Genetic reagent (D. simulans)

Drosophila simulans

13

  

Genetic reagent (D. mojavensis)

Drosophila mojavensis

13

  

Genetic reagent (D. virilis)

Drosophila virilis

13

  

Genetic reagent (D. melanogaster)

UAS-dsxM2RNAi

This study

 

Described below

Genetic reagent (D. melanogaster)

UAS-flag-dsxM2

This study

 

Described below

Genetic reagent (D. melanogaster)

UAS-flag-dsxF

35

 

Described below

Genetic reagent (D. melanogaster)

UAS-myc-dsxM

35

 

Described below

Software,algorithm

LaserGene

DNAStar

http://www.dnastar.com/

 

Software,algorithm

Clustal Omega

EMBL-EBI

https://www.ebi.ac.uk/Tools/msa/clustalo

 

Software,algorithm

ImageJ

ImageJ National Institutes of Health

https://imagej.nih.gov/ij/

 

Software,algorithm

Prism 8

GraphPad

https://www.graphpad.com/

 

Software,algorithm

Integrative Genomics Viewer

37

https://www.igv.org/

 
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