Table 3 Primers used for qPCR experiments.

From: A male-specific doublesex isoform reveals an evolutionary pathway of sexual development via distinct alternative splicing mechanisms

 

Sequences (5′–3′)

actin

Forward: CAGGCGGTGCTTTCTCTCTA

Reverse: AGCTGTAACCGCGCTCAGTA

dsxM

Forward: GAAGAGGCTTCCCGGCGAAT

Reverse: GGACAAATCTGTGTGAGCGG

dsxF

Forward: TTCCGCTATCCTTGGGAGCT

Reverse: CATCCACATTGCCGCGTTGT

dsxM2

Forward: GCCGATCTCAGTTTCCGTCA

Reverse: TCACTGATCAACAGACTTAC

yp2

Forward: TGGGTCAATCCACGTGAAGT

Reverse: ACAATGTAGCCCCTGATCTG

yp3

Forward: GAAGCCGACCAAGTGGCTGA

Reverse: TCCAGACGGGCACATTGCTC

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