Fig. 3: Using See-N-Seq to isolate complex cell phenotypes for RNA sequencing. | Communications Biology

Fig. 3: Using See-N-Seq to isolate complex cell phenotypes for RNA sequencing.

From: See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity

Fig. 3

a Schematic of an immunological synapse formed between Jurkat and Raji cell using Staphylococcus aureus enterotoxin E (SEE) superantigen. b Micrographs of an immunological synapse formed between Jurkat cell (orange) and SEE treated-Raji cells (green). TCR is identified using anti-CD3 (blue) localized at the interface of the two cells. Scale bar = 5 µm. c Micrographs of a non-synapsing Jurkat-Raji cell pair. Scale bar = 5 µm. d Jurkat-Raji cell pair in B isolated via See-N-Seq. Scale bar = 25 µm. e Micrographs of sequenced cells or cell pairs including single Jurkat (Jsc) and Raji (Rsc) cells; synapsing cells at 0, 4 and 24 h (S0sc, S4sc, S24sc); as well as non-synapsing cells at 0, 4 and 24 h (NS0sc, NS4sc, NS24sc). Scale bar = 5 µm.

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