Fig. 4: Differential gene expression data on Jurkat-Raji immunological synapse cell pairs and controls. | Communications Biology

Fig. 4: Differential gene expression data on Jurkat-Raji immunological synapse cell pairs and controls.

From: See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity

Fig. 4

a–h Mean-Divergence plots comparing transcriptomes from Jurkat single cells (Jsc); Raji single cells (Rsc); synapsing cell pairs at 0, 4, and 24 h (S0sc, S4sc, S24sc); non-synapsing cell pairs at 0, 4, and 24 h (NS0sc, NS4sc, NS24sc). X-axis shows average log-CPM (counts per million). Y-axis shows log-fold-change between tested groups. Highlights indicate upregulated (red) and downregulated (blue) genes with p < 0.05. a Jsc vs. Rsc. b Concatenated Jsc and Rsc transcriptomes vs. NS0sc. c S0sc vs. NS0sc. d Comparing two randomly selected groups of S4sc. e S0sc vs. S4sc. f S0sc vs. synapse at 24 h group 1 (S24scG1). g S0sc vs. synapse at 24 h group 2 (S24scG2). h S24scG1 vs. S24scG2. i Heatmap showing log-CPM of putative genes for T helper cell type 1 (Th1) and type 2 (Th2).

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