Table 2 List of sequences.

From: See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity

UMI-dT sequence

AAGCAGTGGTATCAACGCAGAGTACNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTT*(V)*(N)

Template switching oligo

AAGCAGTGGTATCAACGCAGAGTGAATrGrGrG

Primer for LD-PCR

AAGCAGTGGTATCAACGCAGAGT

Custom READ 1

GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC

Custom i5 index read primer

GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGC

Custom index-P5 adapter

AATGATACGGCGACCACCGAGATCTACAC [i5] GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGT*A*C

Index i5 1 [i501]

TAGATCGC

Index i5 2 [i502]

CTCTCTAT

Index i5 3 [i503]

TATCCTCT

Index i5 4 [i504]

AGAGTAGA

Index i5 5 [i505]

GTAAGGAG

Index i5 6 [i506]

ACTGCATA

Index i5 7 [i507]

AAGGAGTA

Index i5 8 [i508]

CTAAGCCT

Index i5 9 [i509]

CGTCTAAT

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