Fig. 2: STRIPAK complex disruption impairs GF-induced collagen I invasion.
From: Cooperation of Striatin 3 and MAP4K4 promotes growth and tissue invasion
a Invasion and representative images of 3D spheroid invasion assay (SIA) of DAOY cells transfected with 10 nM of the indicated siRNA ± GFs (EGF: 30 ng/ml, bFGF: 100 ng/ml). Invasion is calculated as sum of the distances of each migrating cell from the center of the spheroids (n = 4, means ± SEM). Scale bar: 300 µm. b SIA of UW228 cells transfected with 10 nM of the indicated siRNA ± 100 ng/ml bFGF (n = 4, means ± SEM). Scale bar: 300 µm. c SIA and representative images of DAOY cells ± 100 ng/ml bFGF and ± increasing concentrations of PP2A inhibitor LB-100 (n = 3, means ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA). Scale bar: 300 µm. See also Fig. S3. Source data for quantifications shown in (a-c) are available in Supplementary Data 6.
