Fig. 3: Analysis of the ginsenoside Rg2 production in a engineered Saccharomyces cerevisiae strain.

a HPLC analyses of Rg2, Rh1, PPT and PPD production in yeast strains Rg2-01, Rg2-02, Rg2-03, and Rg2-04. The PPT chassis strain PPT-10 was used as a control. Mixed samples of Rf, Rh1, Rg2, PPT and PPD were monitored as standards. b Quantitative analysis of Rg2 and its related intermediates Rh1, PPT, and PPD in yeast strains Rg2-01, Rg2-02, Rg2-03, and Rg2-04. Genetic modification of each strain was drawn under the column, “+” represent the strain possess the corresponding engineering, while “−” represent the corresponding engineering are missing the strain. All data represent the mean of n = 3 biologically independent samples and error bars show standard deviation.