Fig. 3: IAV and SARS-CoV-2 exhibit different infection kinetics in tracheobronchial and alveolar ALI tissue equivalents. | Communications Biology

Fig. 3: IAV and SARS-CoV-2 exhibit different infection kinetics in tracheobronchial and alveolar ALI tissue equivalents.

From: Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents

Fig. 3

Tracheobronchial and alveolar ALI tissues were infected with IAV pH1N1 or PR8 at approx. MOI of 0.1, and SARS-CoV-2 at MOI of 1 (fixed tissue samples shown) or as indicated in titer plots. Apical washes were collected and tissues fixed at 24, 48, 72 and 144 hpi. a Tracheobronchial and (b) alveolar ALI tissues were stained with anti-(IAV) NP and anti-SARS-CoV-2 to label infected cells (shown in green) as well as the nuclear dye Hoechst (blue). c Production of infectious virus from the apical chamber of tracheobronchial or alveolar ALI tissues after exposure to pH1N1 (MOI of ~0.1), PR8 (MOI of ~0.1), or SARS-CoV-2 (MOI of ~0.1 and ~1 for alveolar tissues; MOIs of ~0.1, ~1, ~3, and ~10 for tracheobronchial tissues) at 24, 48, 72, or 144 hpi. IAV titers were measured using a focus forming unit assay on LLC-MMK2 SIAT1 cells, SARS-CoV-2 was measured using plaque assay on Vero E6 cells and expressed as total FFU (IAV) or PFU (SARS-CoV-2)/tissue. Scale bar is 100 μm and 200 μm in IAV and SARS-CoV-2 infected tissues, respectively. Data are represented as M ± SD for a minimum of n = 3 independent experiments/biological replicates except for the data from SARS-CoV-2 infected tracheobronchial tissues at 144 hpi MOI = 3 and MOI = 10 that has n = 1.

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