Fig. 7: Alveolar and tracheobronchial ALI tissues produce tissue-specific chemokines and cytokines in response to IAV and SARS-CoV-2 infection. | Communications Biology

Fig. 7: Alveolar and tracheobronchial ALI tissues produce tissue-specific chemokines and cytokines in response to IAV and SARS-CoV-2 infection.

From: Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents

Fig. 7

Basal compartment media were collected from tracheobronchial (left two panels) or alveolar (right two panels) ALI tissues at indicated time-points and analyzed for cytokine and chemokine secretion by a custom Luminex assay: (a) Chemokines (CXCL10/IP-10, CCL2/MCP-1, CCL3/MIP-1α, IL-8); (b) Interferons (IFN-α and IFN-γ); and (c) Other cytokines (IL-6, IL-10, IL-1RA). IAV infected tissues (MOI of ~0.1) are represented in shades of teal, where light teal shows infection with the IAV pH1N1 strain and dark teal shows infection with the IAV PR8 strain, whereas SARS-CoV-2 infected tissues are represented in shades of purple, with progressing color from low MOI (~1) to high MOI (~10). All measurements on y axis are in pg/ml. Data are represented as M ± SEM for a minimum of n = 3 independent experiments and/or biological replicates except for the data from SARS-CoV-2 infected tracheobronchial tissues at 144 hpi MOI = 10 that has n = 1 and the SARS-CoV-2 set matched Mock alveolar tissues at 72hpi that has n = 2; Student t test of IAV or SARS-CoV-2 infected tissues vs. uninfected controls at each timepoint: *p  <  0.05, **p  <  0.005, ***p  <  0.0005, ****p  <  0.00005.

Back to article page