Fig. 6: Cry78Aa share common characteristics of aerolysin-type pore-forming toxins.

a The putative membrane-spanning region (MSR). The NTD and CTD are shown in color of light blue and green, respectively. The MSR located at the CTD and is enclosed in a black frame, colored in yellow, and the residues shown as lines (color representations: carbon, yellow; nitrogen, blue; oxygen, red; sulfur, orange). The ribbon representation is shown in partial transparency for clarity. b Close-up view of the alternating hydrophobic and hydrophilic residues in the MSR. The hydrophobic residues are shown as sticks and labeled. The hydrophilic residues are omitted for clarity. Amino acids sequence of the MSR is listed below and the hydrophobic residues are underlined. Color representation is the same as diagram (a). c Close-up view of the serine/threonine patches intra or in vicinity of the MSR. All the hydrogen bond interactions intra MSR or between MSR and other residues are presented. The yellow dashed lines represent hydrogen bonds. Serine and threonine participate in the interactions are shown as sticks, with category and number labeled beside. Other residues participate in the interactions are shown as lines (color representations: carbon, yellow for MSR and green/light blue for CTD/NTD; nitrogen, blue; oxygen, red; sulfur, orange). d Detailed depiction of the hydrogen bonds presented in diagram (b). Most hydrogen bonds consisted of residues that occurred in serine/threonine patches.