Fig. 3: PYK2 and FAK KFL dimerise.
From: PYK2 senses calcium through a disordered dimerization and calmodulin-binding element
a, b SEC-MALS elution profiles on purified proteins. The Mw deduced for each peak is given. The additional lines show the molar mass distribution. c, d MST and e, f fluorescence anisotropy (FA) studies. The red star indicates the fluorescently labelled protein. MBP fusions were used to increase the Mw of the unlabelled molecule in FA. g Summary table, also including data derived from Supplementary Fig. 7a–f). The single square bracket indicates that the Kd in FA was established between fluorescently labelled protein and MBP-fused unlabelled protein. D: dimer; M: monomer. Binding experiments are represented as (mean ± SD, n = 3).
