Table 1 List of the primers used for cloning.
From: PYK2 senses calcium through a disordered dimerization and calmodulin-binding element
Name | Primers | |
|---|---|---|
MBP–FAK764–845 | Forward | CCAGGGAGCAGCCTCGATGCTGGAAGTTCTGTTCCAGGG |
Reverse | GCAAAGCACCGGCCTCGTCAGTCGATAGAACCACGAGACAG | |
6×His–FAK764–845 | Forward | ATTGGATCCCAGGCGTCTCTGCTGGACCAGACCGAC |
Reverse | ATTCTCGAGCTAGTCGATAGAACCACGAGACAGACG | |
MBP–FAK776–841 | Forward | CCAGGGAGCAGCCTCGATGCTGGAAGTTCTGTTCCAGGGTCC |
Reverse | GCAAAGCACCGGCCTCGTCAACGAGACAGACGAACGTCCGG | |
6×His–FAK776–841 | Forward | ATTGGATCCCGTCCACAGGAAATCGCTATGTGG |
Reverse | ATTCTCGAGCTAACGAGACAGACGAACGTCCGGTTTC | |
6×His–FAK804–832 | Forward | ATTGGATCCCCGACCCACCTGATGGAGGAGCGT |
Reverse | ATTCTCGAGCTAGAAGCGTTCTTCTTTTTCCAGCCAA | |
MBP–PYK2728–839 | Forward | CCAGGGAGCAGCCTCGACCAACCTGCTGGCGCCGAAAC |
Reverse | GCAAAGCACCGGCCTCGTCAAGATTTGTCGTTCATGTAAACCATCGGGTC | |
6xHis–PYK2728–839 | Forward | ATTGGATCCACCAACCTGCTGGCGCCGAAACTC |
Reverse | ATTCTCGAGTCAAGATTTGTCGTTCATGTAAACCATC | |
MBP–PYK2761–839 | Forward | CCAGGGAGCAGCCTCGAACTCTCTGCACACTCCGCCGC |
Reverse | GCAAAGCACCGGCCTCGTCAAGATTTGTCGTTCATGTAAACCATCGGG | |
6×His–PYK2761–839 | Forward | ATTGGATCCAACTCTCTGCACACTCCGCCG |
MBP–PYK2789–839 | Forward | CCAGGGAGCAGCCTCGTCTCGTGAAGAAGCGCAGCAGCT |
Reverse | GCAAAGCACCGGCCTCGTCAGTCGTTCATGTAAACCATCGGG | |
6×His–PYK2789–839 | Forward | ATTGGATCCTCTCGTGAAGAAGCGCAGCAGCTG |
