Fig. 4: Compound having similar mechanisms of action cluster in morphological space. | Communications Biology

Fig. 4: Compound having similar mechanisms of action cluster in morphological space.

From: Integrating cell morphology with gene expression and chemical structure to aid mitochondrial toxicity detection

Fig. 4

Principal Component Analysis of 542 compounds in 110-dimensional Cell Painting feature space. Certain compounds clustered further away from the distribution of majority of compounds having similar mechanisms of actions such as of microtubule destabilizers, or compounds inducing apoptotic signalling cascades, compounds causing oxidative stress due to GSH depletion or those that are inhibitors of plasma membrane Na+ pump (all of which reduce mitochondrial membrane potential). Cluster I (microtubule destabilisers): rotenone, albendazole, parbendazole, mebendazole, nocodazole, fenbendazole, colchine, paclitaxel and podophyllotoxin; Cluster II (inhibitors of plasma membrane Na+ pump): ouabain and digoxin; Cluster III (caspase activation and GSH depletion): devazepide, lovastatin, simvastatin; and Cluster II (trigger the release of cytochrome c into the cytosol): mevastatin, prazosin, and raloxifene. The Figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license.

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