Fig. 1: Stronger interaction of CT-α-syn with membrane. | Communications Biology

Fig. 1: Stronger interaction of CT-α-syn with membrane.

From: C-terminal truncation modulates α-Synuclein’s cytotoxicity and aggregation by promoting the interactions with membrane and chaperone

Fig. 1

a Primary structure schematic of FL- and CT-α-syn. b Cartoon of coflotation assay. c SDS-PAGE of fractions post centrifugation using POPC/CL (7:3 mole ratio) liposomes. FL- and CT-α-syn were run on SDS-PAGE independently. d The proportion of lipid-bound protein from SDS-PAGE data. Uncertainties are the standard error of the mean from three independent trials. e Overlaid 1H-15N HSQC spectra of 0.12 mM 15N-enriched FL- and CT-α-syn in the absence (black) and presence (red) of 12 mM POPC/CL (7:3 mole ratio) liposomes and residue-resolved attenuation (I/I0) of FL- (black circles) and CT-α-syn (red circles) upon adding liposomes. Values <1.0 indicate interactions.

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