Fig. 4: High-dose single-digit microsecond exposure of a protein sample.
From: An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins
a Intact mass analysis of exposed cyt c shows a significant amount of modification within 10 μs. b Total ion chromatogram of exposed and trypsin digested cyt c using a 50 μm liquid jet and single-digit microsecond exposure. The arrow indicates the progressive increase in modified peptides with increasing exposure time. c Total ion chromatogram of exposed and trypsin digested cyt c using 200 μm ID capillary using a standard flow setup, which used 150 μm Al attenuation to prevent sample damage and glass capillary breakage. The arrow indicates the significant decrease in unmodified peptides with increasing exposure time. d Extracted ion chromatogram of the unmodified (390.21 m/z, z = 3) and +16 Da modified (395.54 m/z, z = 3) cyt c peptide 28TGPNLHGLFGR38 using 50 μm liquid jet and single-digit microsecond exposure. e Extracted ion chromatogram of the same peptide as that in D, using 200 μm ID capillary in the standard flow setup with 150 μm Al attenuation. Numerical source data are available in Supplementary Data 2.
