Fig. 6: Microglia dynamics in the rapid diffusion phase.
From: Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization
a Repetitive in vivo imaging in the same FOV after occlusion shows a resolving microglia cluster over time. White arrows indicate microglia of round shape with few processes. Yellow arrows indicate microglia with asymmetric processes. Purple arrows indicate microglia with well-proportioned ramified process. Scale bar, 40 μm. b Persistent relative intensity variance at different time points from the inner to the outer area as shown in Fig. 2b (n = 3 mice). Data are plotted as mean ± SEM. c The number variance of microglia from day 1 to day 5 after occlusion (n = 3 mice). Data are plotted as mean ± SEM. d Images showing difference in the number and morphology of microglia at day 1 and day 5 after occlusion. Compared with that at day 1 (yellow arrows), the symmetry of microglia process is significantly improved at day 5 (purple arrows). Scale bar, 20 μm. e Quantification of T-index at different time points (n = 3 mice, ** P < 0.01, ****P < 0.0001 versus T-index on day 1, one-way ANOVA followed by Tukey’s multiple comparison). Error bars, mean and SEM. f Images showing proliferative microglia during repeated imaging in vivo. Scale bar, 20 μm. g Representative images of immunostaining for Brdu (cyan) and DAPI (blue) in the contralateral and ipsilateral cortex of vessel occlusion CX3CR1-GFP mouse in the rapid diffusion phase. Scale bar, 40 μm. h Quantitative analysis of Brdu+GFP+ cells in the contralateral and ipsilateral cortex as shown in g (n = 5 mice, **P < 0.01, paired t test). Error bars, mean and SEM.
