Fig. 8: Microglia recover to resting state in the chronic effect phase.
From: Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization
a Representative in vivo image in the chronic phase. Scale bar, 40 μm. b Quantification of relative fluorescence intensity in the chronic phase from the inner to the outer area as shown in Fig. 2b (n = 3 mice, one-way ANOVA followed by Fisher’s LSD multiple comparison). c Time-course analysis of microglia number in the chronic phase (n = 3 mice, one-way ANOVA followed by Fisher’s LSD multiple comparison). d Quantification of T-index in the chronic phase (n = 3 mice, one-way ANOVA followed by Tukey’s multiple comparison). e Representative microglial morphology images in vivo of control and vessel occlusion mice. Scale bar, 30 μm. f Sholl analysis of microglial morphology in vivo in control and vessel occlusion mice (n = 3 mice, Two-way ANOVA). g–i Quantification of cell morphometry, including soma size (g), total number of process (h) and process length (i) of microglia (n = 3 mice, unpaired t test). j Representative images of immunostaining for Iba1 (green), MHCII (cyan) and DAPI (blue) in the contralateral and ipsilateral cortex of vessel occlusion mouse in the chronic effect phase. Scale bar, 40 μm. k–l Quantitative analysis of MHCII (k) and Iba1 (l) immunostaining intensity as shown in j, n = 4 mice, paired t test. Data are presented as mean and SEM, ns indicates no significant difference.
