Fig. 4: Ag-binding and immune complex formation are affected by pH and differ between IgG1s and Fc-fusions. | Communications Biology

Fig. 4: Ag-binding and immune complex formation are affected by pH and differ between IgG1s and Fc-fusions.

From: Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life

Fig. 4

a–e SPR sensorgrams showing ABTs (100 nM) injected over and binding to immobilized cognate Ags (~300 RU) at either pH 5.5 or 7.4. Analytical SEC of ABTs preincubated with increasing amounts of their cognate Ag at either (f–j) pH 5.5 or (k–o) 7.4 before injection. Color labeling a–d; Ab-Ag injected at pH 5.5 (red) and at pH 7.4 (black). Color labeling f–o; Ab only (black), Ab:Ag 1:1 (red), Ab:Ag 1:2 (teal), Ab:Ag 1:4 (dark purple).

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