Fig. 5: Ag-binding alters the Fc-FcRn interaction. | Communications Biology

Fig. 5: Ag-binding alters the Fc-FcRn interaction.

From: Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life

Fig. 5

a, c, e, g, i Sensorgrams showing injection of 15.62 nM ABTs preincubated with increasing amounts of cognate Ags at pH 5.5 and injected over immobilized (~100 RU) site-specifically biotinylated FcRn at pH 5.5. b, d, f, h, j Elution profiles from FcRn chromatography of ABTs preincubated with increasing amounts of cognate Ags at pH 5.5 shown as relative fluorescence intensity and as a function of pH (plotted on the right Y-axis, indicated by a stapled line) Color labeling; Ab only (black), Ab:Ag 1:1 (red), Ab:Ag 1:2 (teal), Ab:Ag 1:4 (dark purple).

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