Fig. 5: TDP-43 upregulates ABHD2 through enhancing the mRNA stability of ABHD2.

a RIP assay to detect the interaction between TDP-43 and ABHD2 mRNA in MHCC97H cells. The data was normalized to the Input. b The mRNA stability of ABHD2 was analyzed by qRT-PCR in SMMC-7721 cells upon TDP-43 deletion. The cells were treated with ActD for the indicated time points after transient transfection for the indicated siRNAs. c The mRNA stability of ABHD2 was analyzed by qRT-PCR in MHCC97H-KD-TDP-43 and MHCC97H-Control cells. The cells were treated with ActD for the indicated time points. Luciferase reporter gene assay of ABHD2 3ʹUTR activity upon TDP-43 overexpression (d) or knockdown (e). The cells were transiently transfected with the indicated plasmids or siRNAs. f Schematic representation of ABHD2 3ʹUTR constructed on pGL3-Control plasmid. WT represents the wild-type plasmid of ABHD2 3ʹUTR; Del-1 represents the WT plasmid without the binding site1 (UG-rich sequence1, 6704–6709 refer to NM_007011.8); Del-2 represents the WT plasmid without the binding site2 (UG-rich sequence2, 7122–7129 refer to NM_007011.8). Luciferase reporter gene assay of ABHD2 3ʹUTR activities upon TDP-43 overexpression (g) or knockdown (h). The HEK 293 T cells were transiently transfected with WT, Del-1 or Del-2 accompanied with the indicated plasmids or siRNAs. The values in the graphs represent the mean of three biologically independent experiments. Error bars represent ± s. d. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student’s t test.