Fig. 6: Lipid metabolism modulator ABHD2 contributes to the malignant behaviors of HCC. | Communications Biology

Fig. 6: Lipid metabolism modulator ABHD2 contributes to the malignant behaviors of HCC.

From: TDP-43 upregulates lipid metabolism modulator ABHD2 to suppress apoptosis in hepatocellular carcinoma

Fig. 6

a Analysis of free fatty acid (FFA) levels in SMMC-7721 (left) and MHCC97H (right) cells which were transiently transfected with si-Control or si-ABHD2s. Quantitative analysis (b) and fluorescence images (c) of reactive oxygen species (ROS) in Huh-7 and MHCC97H cells transiently transfected with the displayed plasmid or siRNAs. Scale bar: 200 µm. d–f Flow cytometry analysis of apoptosis in SMMC-7721 and MHCC97H cells transfected with si-Control or si-ABHD2s for 48 h. The statistical graphs of apoptotic rate were shown in (f). g Western blot analysis of protein levels of ABHD2, Bax, C-caspase3, p53, and Bcl-2 in SMMC-7721 and MHCC97H cells transiently transfected with si-ABHD2s or si-Control. The values in the graphs represent the mean of three biologically independent experiments. Error bars represent ± s. d. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student’s t-test.

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