Fig. 3: mTOR inhibition of LPS-stimulated monocytes promotes an inflammatory transcriptional program.
From: mTOR regulation of metabolism limits LPS-induced monocyte inflammatory and procoagulant responses
a–d Monocytes from six independent donors were pretreated with an mTORi (AZD2014 at 5 μM, 6 h) or DMSO and stimulated with LPS (1 ng, 6 h) for bulk RNA-seq analysis. Select HALLMARK gene sets identified as differentially regulated through GSEA, presenting expression data comparing LPS-stimulated monocytes to unstimulated monocytes (a) and mTORi-treated, stimulated monocytes to LPS-stimulated monocytes without mTORi pretreatment (b)81. For complete HALLMARK GSEA, see also Supplementary Fig. 4. c, d Differential expression of genes of interest. c Expression normalized by z score; genes that did not reach significance are noted in light gray. d Significantly upregulated (red) or downregulated (blue) genes determined using an FDR adjusted p value cut-off of 0.05. Top 50 genes by FDR adjusted p value labeled.
