Fig. 5: mTOR inhibition potentiates surface expression of tissue factor on monocytes from uninfected and SIV-infected macaques in an equivalent manner.
From: mTOR regulation of metabolism limits LPS-induced monocyte inflammatory and procoagulant responses
a, b Cryopreserved PBMCs from six uninfected and six chronically SIVmac239-infected rhesus macaques were pretreated with an mTORi (AZD2014 at 5 μM, 6 h) or DMSO and stimulated with LPS (1 ng, 12 h) prior to staining for flow cytometry. For b, gray dots represent uninfected (SIV−) animals and red dots represent infected (SIV+). Representative flow plots (a) and aggregate (b) represent gating on leukocyte/singlet/live/CD11b+/CD14+. For additional details on gating strategy see Supplementary Fig. 6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p values calculated using a two-way ANOVA and Sidak’s multiple comparisons. Error bars represent mean ± SD.
