Fig. 5: Adult IRSp53 re-expression decreases excitatory synaptic transmission and increases inhibitory synaptic transmission in IRSp53-cKO mice. | Communications Biology

Fig. 5: Adult IRSp53 re-expression decreases excitatory synaptic transmission and increases inhibitory synaptic transmission in IRSp53-cKO mice.

From: Adult re-expression of IRSp53 rescues NMDA receptor function and social behavior in IRSp53-mutant mice

Fig. 5

a Examples of mEPSC traces in layer 5 pyramidal neurons from the prelimbic region of the mPFC in WT and IRSp53-cKO mice that received retro-orbital sinus injections of PHP.eB-IRSp53 or PHP.eB-EGFP (control) at postnatal week 8 and underwent mEPSC measurements at postnatal week 12. b, c Decreased frequency, but not amplitude, of mEPSCs in layer 5 pyramidal neurons of WT and IRSp53-cKO mice injected with PHP.eB-EGFP or PHP.eB-IRSp53 (n = 15 neurons from 3 mice [WT-EGFP], 15, 4 [WT-IRSp53], 9, 5 [IRSp53-cKO-EGFP], and 18, 4 [IRSp53-cKO-IRSp53], two-way ANOVA). d Examples of mIPSC traces in layer 5 pyramidal neurons from the prelimbic region of the mPFC in WT and IRSp53-cKO mice that received retro-orbital sinus injections of PHP.eB-IRSp53 or PHP.eB-EGFP (control) at postnatal week 8 and underwent mIPSC measurements at postnatal week 12. e, f Increased amplitude, but not frequency, of mIPSCs in layer 5 pyramidal neurons of WT and IRSp53-cKO mice injected with PHP.eB-EGFP or PHP.eB-IRSp53 (n = 8, 2 [WT-EGFP], 9, 2 [WT-IRSp53], 7, 2 [IRSp53-cKO-EGFP], and 12, 2 [IRSp53-cKO-IRSp53], two-way ANOVA). Significance values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001, or ns (not significant, p > 0.05). Error bars represent the standard errors of means.

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