Fig. 1: Crystal structure of ChbG from Klebsiella pneumoniae (kpChbG). | Communications Biology

Fig. 1: Crystal structure of ChbG from Klebsiella pneumoniae (kpChbG).

From: Crystal structure of ChbG from Klebsiella pneumoniae reveals the molecular basis of diacetylchitobiose deacetylation

Fig. 1

a Catalytic reaction of ChbG. b Size-exclusion chromatography (SEC) of Ni2+-affinity purified ChbG. Loaded fractions are indicated by the horizontal black bar. Inset: SDS-PAGE assessment of purity. M: size marker and B: before SEC loading. c ESI-MS analysis of deacetylation of N,N’-diacetylchitobiose ((GlcNAc)2) to N-acetyl-β-glucosaminyl-glucosamine (GlcN-GlcNAc) by kpChbG. d Cartoon representation of two kpChbG molecules (molecule A and B) presented in an asymmetric unit. e Superposition of the structures in one asymmetric unit. f Rainbow-colored cartoon representation of monomeric kpChbG. The polypeptide chain from the N-terminus to the C-terminus is colored blue to red. Helices and sheets are labeled α and β, respectively. ACT represents acetate and Ion is the metal ion. g Putty representation showing B-factor distribution in the order of B-factor values using rainbow colors (red to violet).

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