Fig. 3: Acute L-DOPA treatment rescues PD-associated motor defects, but prolonged L-DOPA treatment induces AIMs by PD model flies. | Communications Biology

Fig. 3: Acute L-DOPA treatment rescues PD-associated motor defects, but prolonged L-DOPA treatment induces AIMs by PD model flies.

From: Discovery of levodopa-induced dyskinesia-associated genes using genomic studies in patients and Drosophila behavioral analyses

Fig. 3

a Experimental scheme to assay single fly trajectories upon acute L-DOPA diets intercalated with control diets. Thick green and gray arrows indicate time periods of L-DOPA and control diets, respectively. Black arrows indicate video recordings of the fly movement. b Representative trajectories of a control (PINK1RV) and a PD model fly (PINK1B9) upon acute L-DOPA diets. Abnormal locomotor patterns are highlighted in red circles. c, d Comparisons of the quantified speed (c) and AIM scores (d) of the control and PD model flies upon acute L-DOPA diets. N = 6. ****p  < 0.0001; ***p < 0.001; n.s., not significant (p > 0.05) by two-way ANOVA Tukey’s multiple comparison test and are presented as means ± SEM. p < 0.05 was considered statistically significant. e Experimental scheme to assay single fly trajectories upon chronic L-DOPA diets intercalated with control diets. Thick green and gray arrows indicate time periods of L-DOPA and control diets, respectively. Black arrows indicate video recordings of the fly movement. f Representative trajectories of a PINK1RV and a PINK1B9 fly upon chronic L-DOPA diet. Abnormal locomotor patterns are highlighted in red circles. g, h Comparisons of the quantified speed (g) and AIM scores (h) of the control and PD model flies upon chronic L-DOPA diet. N = 6. ****p < 0.0001; **p < 0.01; n.s., not significant (p > 0.05) by two-way ANOVA Tukey’s multiple comparison test. Data are presented as means ± SEM. p < 0.05 was considered statistically significant.

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