Fig. 7: In vitro experiments showing that ALDH1A3 is the most expressed isoform in GL261 murine glioma cells and both fluorescent substrates enter and label the same cells. | Communications Biology

Fig. 7: In vitro experiments showing that ALDH1A3 is the most expressed isoform in GL261 murine glioma cells and both fluorescent substrates enter and label the same cells.

From: Curcumin-based-fluorescent probes targeting ALDH1A3 as a promising tool for glioblastoma precision surgery and early diagnosis

Fig. 7

a Results of quantitative rtPCR showing the levels of expression of ALDH1A1 (1a1), ALDH1A2 (1a2) and ALDH1A3 (1a3) in murine GL261 cells. ALDH1A3 is significantly more expressed than the other isoforms. b Living GL261 cells were analyzed through an ImageStreamX MarkII using two channels: brightfield (Ch 01) and fluorescence (Ch 02) after 1 h incubation in probe 10, cells were thoroughly washed in PBS and analyzed. Two different representative cells are shown. From above, row 1 brightfield images (Ch 01), row 2 fluorescent images (Ch 02). “In Focus Cells” were identified based on the “Gradient Root Mean Square (RMS) Contrast Feature” that captures in focus images of cells identified by high normalized pixel intensity gradient (RMS values) derived from Ch 01; then, a scatter plot of the “Aspect Ratio Feature” vs. brightfield “Area Feature” was used to identify single cells (singlets) from debris or cell clumps based on high aspect ratio and low area value. c Fluorescence intensity variations are shown in the histogram and dot plot (Area vs. Intensity). Scale bars: 5 µm are the same in all images.

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