Fig. 8: In vivo labeling by the fluorescent ALDH1A3 substrates of GL261 cells growing and infiltrating the host brain 6 days after the initial inoculum. | Communications Biology

Fig. 8: In vivo labeling by the fluorescent ALDH1A3 substrates of GL261 cells growing and infiltrating the host brain 6 days after the initial inoculum.

From: Curcumin-based-fluorescent probes targeting ALDH1A3 as a promising tool for glioblastoma precision surgery and early diagnosis

Fig. 8

a–f Confocal images showing the same coronal section of a tumor-bearing brain in an animal. injected i.p. with Probe 10 for 6 h. In all images the area boxed is the same and it is shown at higher magnification in d–f. In a cell nuclei are stained by DAPI, in 6b, GL261 cells containing Probe 10 fluorescence in green. Most of the fluorescence comes from glioma cells growing in the left striatum and adjacent structures that have taken up and metabolized Probe 10. Scale bars: 25 μM. In 6c, the image shows the double fluorescence of DAPI and Probe 10 together. d–f show confocal images showing at higher magnification the area boxed in 6a–c. Fluorescence of Probe 10 is mostly contained in the cytoplasm. Scale bars: 25 μM. g–h Confocal images showing, under different fluorescence conditions, the same coronal section of a tumor-bearing brain in an animal i.p. injected with compound Probe 11 that does not penetrate to any appreciable level the tumor cells in vivo, despite penetrating GL261 cells in vitro. Scale bars: 25 μM. i Confocal image under excitation and filtering conditions appropriate both for DAPI and Probe 11, no fluorescence due to Probe 11 is visible despite the abundance of tumor cells.

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